Article Publish Status: FREE
Abstract Title:

Brazilian Red Propolis: Extracts Production, Physicochemical Characterization, and Cytotoxicity Profile for Antitumor Activity.

Abstract Source:

Biomolecules. 2020 05 6 ;10(5). Epub 2020 May 6. PMID: 32384801

Abstract Author(s):

Felipe Mendes de Andrade de Carvalho, Jaderson Kleveston Schneider, Carla Viviane Freitas de Jesus, Luciana Nalone de Andrade, Ricardo Guimarães Amaral, Jorge Maurício David, Laíza Canielas Krause, Patrícia Severino, Cleide Mara Faria Soares, Elina Caramão Bastos, Francine Ferreira Padilha, Silvana Vieira Flores Gomes, Raffaele Capasso, Antonello Santini, Eliana Barbosa Souto, Ricardo Luiz Cavalcanti de Albuquerque-Júnior

Article Affiliation:

Felipe Mendes de Andrade de Carvalho


Brazilian red propolis has been proposed as a new source of compounds with cytotoxic activity. Red propolis is a resinous material of vegetal origin, synthesized from the bees of the Appis mellifera family, with recognized biological properties. To obtain actives of low polarity and high cytotoxic profile from red propolis, in this work, we proposed a new solvent accelerated extraction method. A complete 2factorial design was carried out to evaluate the influence of the independent variables or factors (e.g., temperature, number of cycles, and extraction time) on the dependent variable or response (i.e., yield of production). The extracts were analyzed by gas chromatography coupled with mass spectrometry for the identification of chemical compounds. Gas chromatography analysis revealed the presence of hydrocarbons, alcohols, ketones, ethers, and terpenes, such as lupeol, lupenone, and lupeol acetate, in most of the obtained extracts. To evaluate the cytotoxicity profile of the obtained bioactives, the 3-(4,5-dimethyl-2-thiazole)-2,5-diphenyl-2--tetrazolium bromide colorimetric assay was performed in different tumor cell lines (HCT116 and PC3). The results show that the extract obtained from 70°C and one cycle of extraction of 10 min exhibited the highest cytotoxic activity against the tested cell lines. The highest yield, however, did not indicate the highest cytotoxic activity, but the optimal extraction conditions were indeed dependent on the temperature (i.e., 70 °C).

Study Type : In Vitro Study

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