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Article Publish Status: FREE
Abstract Title:

Bromelain Inhibits SARS-CoV-2 Infection in VeroE6 Cells.

Abstract Source:

bioRxiv. 2020 Sep 16. Epub 2020 Sep 16. PMID: 32995771

Abstract Author(s):

Satish Sagar, Ashok Kumar Rathinavel, William E Lutz, Lucas R Struble, Surender Khurana, Andy T Schnaubelt, Nitish Kumar Mishra, Chittibabu Guda, Mara J Broadhurst, St Patrick M Reid, Kenneth W Bayles, Gloria E O Borgstahl, Prakash Radhakrishnan

Article Affiliation:

Satish Sagar

Abstract:

: Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). The initial interaction between Transmembrane Serine Protease 2 (TMPRSS2) primed SARS-CoV-2 spike (S) protein and host cell receptor angiotensin-converting enzyme 2 (ACE-2) is a pre-requisite step for this novel coronavirus pathogenesis. Here, we expressed a GFP-tagged SARS-CoV-2 S-Ectodomain in Tni insect cells. That contained sialic acid-enriched N- and O-glycans. Surface resonance plasmon (SPR) and Luminex assay showed that the purified S-Ectodomain binding to human ACE-2 and immunoreactivity with COVID-19 positive samples. We demonstrate that bromelain (isolated from pineapple stem and used as a dietary supplement) treatment diminishes the expression of ACE-2 and TMPRSS2 in VeroE6 cells and dramatically lowers the expression of S-Ectodomain. Importantly, bromelain treatment reduced the interaction between S-Ectodomain and VeroE6 cells. Most importantly, bromelain treatment significantly diminished the SARS-CoV-2 infection in VeroE6 cells. Altogether, our results suggest that bromelain or bromelain rich pineapple stem may be used as an antiviral against COVID-19.

Highlights: Bromelain inhibits / cleaves the expression of ACE-2 and TMPRSS2Bromelain cleaves / degrades SARS-CoV-2 spike proteinBromelain inhibits S-Ectodomain binding and SARS-CoV-2 infection.

Study Type : In Vitro Study

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