Abstract Title:

Chiral separation of catechins in buckwheat groats and the effects of phenolic compounds in mice subjected to restraint stress.

Abstract Source:

J Agric Food Chem. 2009 Jul 22;57(14):6438-42. PMID: 19601674

Abstract Author(s):

Mitsuru Watanabe, Jun Ayugase

Article Affiliation:

National Agricultural Research Center for Tohoku Region, Morioka, Iwate 020-0198, Japan. [email protected]


This paper investigates the stereochemistry of catechin and epicatechin compounds in buckwheat ( Fagopyrum esculentum Moench) groats and then examines the effects of an orally administered mixture of the phenolic compounds isolated from the groats in restrained mice. Phenolic compounds separated by Sephadex LH-20 chromatography contained catechin and epicatechin compounds with a sugar moiety or esterified with phenolic acid derivatives along with rutin. Other unidentified major compounds in the HPLC chromatogram were also deduced on the basis of the spectrometric data with LC-MS/MS analysis to be catechin or epicatechin compounds. A chiral HPLC separation technique under normal-phase conditions showed that catechin and its derivative compounds in buckwheat groats were (+)-isomers, whereas epicatechin and its derivative compounds were (-)-isomers. Propylene glycol (PPG) or buckwheat groats phenolic compounds (BGP) in PPG (10 or 100 mg/kg of body weight) were administered intragastrically once per day for 3 days to mice, which were then restrained for 24 h. Unrestrained mice were given PPG solution with or without free access to feed and water. Restraint stress induced an elevation in plasma arteriosclerotic index, hepatic total cholesterol, and the amount of thiobarbituric acid-reactive substances (TBARS) in plasma and liver tissues. In contrast, these variables were suppressed in the restrained mice that were given BGP. These results suggest that BGP has in vivo antistress effects against the reactions induced by immobilization in mice.

Study Type : Animal Study

Print Options

Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2022 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.