Article Publish Status: FREE
Abstract Title:

Bufalin Inhibits Proliferation and Induces Apoptosis in Osteosarcoma Cells by Downregulating MicroRNA-221.

Abstract Source:

Evid Based Complement Alternat Med. 2016 ;2016:7319464. Epub 2016 Dec 15. PMID: 28074104

Abstract Author(s):

Jianjun Zhang, Jingjing Sha, Yan Zhou, Kun Han, Yaling Wang, Yang Su, Xiuyi Yin, Haiyan Hu, Yang Yao

Article Affiliation:

Jianjun Zhang


Bufalin, a major component of the Chinese medicine ChanSu, which is prepared from the skin and parotid venom glands of toads, has shown cytotoxicity in several malignant tumors. Here, we reported that bufalin inhibited proliferation and induced mitochondria-dependent apoptosis in U-2OS and Saos-2 osteosarcoma cells with intracellular reactive oxygen species (ROS) production. By microRNA (miR) array analysis and quantitative reverse transcription polymerase chain reaction, we found that miR-221 was downregulated after treatment with bufalin. In accordance with TargetScan prediction and luciferase reporter assay, Bcl2 binding component 3 (BBC3) was the direct target of miR-221. Furthermore, upregulating miR-221 by its MIMIC and suppressing BBC3 by small interfering RNA (siRNA) reversed the effects of bufalin on osteosarcoma cells. Collectively, our data indicate that bufalin inhibits cell proliferation and induces mitochondria-dependent apoptosis in osteosarcoma cells through downregulating miR-221 and triggering BBC3 expression.

Study Type : In Vitro Study

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