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Abstract Title:

Cardioprotective effects of rhamnetin in H9c2 cardiomyoblast cells under H₂O₂-induced apoptosis.

Abstract Source:

J Ethnopharmacol. 2014 May 14 ;153(3):552-60. Epub 2014 Mar 7. PMID: 24607510

Abstract Author(s):

Eun-Seok Park, Jun Chul Kang, Yong Chang Jang, Jong Seok Park, Shin Yi Jang, Dae-Eun Kim, Bokyung Kim, Hwa-Sup Shin

Article Affiliation:

Eun-Seok Park

Abstract:

ETHNOPHARMACOLOGICAL RELEVANCE: Many studies have emphasized that flavonoids, found in various fruits, vegetables, and seeds, as well as tea and red wine, have potential health-promoting and disease-preventing effects. Rhamnetin is a flavonoid that exhibits antioxidant capabilities. However, little is known about its effect on cardiac myocytes under oxidative stress and the underlying mechanisms.

MATERIALS AND METHODS: H9c2 cardiomyoblast cells were subjected to H2O2, to study the protective effect of rhamnetin on cell viability, apoptosis, and ROS production. Signaling proteins related to apoptosis, survival, and redox were analyzed by Western blot. Furthermore, the mRNA expressions of SIRTs were tested by real time-polymerase chain reaction (PCR).

RESULTS: We investigated the protective effects of rhamnetin against H₂O₂-induced apoptosis in H9c2 cardiomyoblasts. Rhamnetin protected cells against H₂O₂-induced cell death without any cytotoxicity, as determined by the XTT assay, LDH assay, TUNEL assay, Hoechst 33342 assay, and Western blot analysis of apoptosis-related proteins. Rhamnetin also enhanced the expression of catalase and Mn-SOD, thereby inhibiting production of intracellular ROS. Furthermore, rhamnetin recovered the H₂O₂-induced decrease in phosphorylation of Akt/GSK-3βand MAPKs (ERK1/2, p38 MAPK, and JNK) and pretreatment with their inhibitors, attenuating the rhamnetin-induced cytoprotective effect. Further studies with real time-PCR and a sirtuin inhibitor showed that cardioprotection by rhamnetin occurred through induction of SIRT3 and SIRT4.

CONCLUSIONS: Taken together, these results suggest that rhamnetin may have novel therapeutic potential to protect the heart from ischemia-related injury.

Study Type : In Vitro Study

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