extract prevents visual impairment by promoting the production of rhodopsin in the retina.
Nutr Res Pract. 2020 Jun ;14(3):203-217. Epub 2020 Jan 28. PMID: 32528628
Dae Won Park
BACKGROUND/OBJECTIVE: , also known as Gotu kola, is a tropical medicinal plant native to Madagascar, Southeast Asia, and South Africa. It is well known to have biological activities, including wound healing, anti-inflammatory, antidiabetic, cytotoxic, and antioxidant effects. The purpose of this study was to determine the efficacy of extracts ofagainst age-related eye degeneration and to examine their physiological activities.
MATERIALS/METHODS: To determine the effects of CA-HE50 (50% EtOH extract) on retinal pigment cells, we assessed the cytotoxicity of CoCland oxidized-A2E in ARPE-19 cells and observed the protective effects of CA-HE50 against-methyl--nitrosourea (MNU)-induced retinal damage in C57BL/6 mice. In particular, we measured factors related to apoptosis and anti-oxidation and the protein levels of rhodopsin/opsin. We also measured glucose uptake to characterize glucose metabolism, a major factor in cell protection.
RESULTS: Induction of cytotoxicity with CoCland oxidized-A2E inhibited decreases in the viability of ARPE-19 cells when CA-HE50 was administered, and promoted glucose uptake under normal conditions (<0.05). In addition, CA-HE50 inhibited degeneration/apoptosis of the retina in the context of MNU-induced toxicity (<0.05). In particular, CA-HE50 at 200 mg/kg inhibited the cleavage of pro-caspase-3 and pro-poly (ADP-ribose)-polymerase and maintained the expressions of nuclear factor erythroid 2-related factor 2 and heme oxygenase-1 similar to normal control levels. Rhodopsin/opsin expression was maintained at a higher level than in normal controls.
CONCLUSION: A series of experiments confirmed that CA-HE50 was effective for inhibiting or preventing age-related eye damage/degeneration. Based on these results, we believe it is worthwhile to develop drugs or functional foods related to age-related eye degeneration using CA-HE50.