Chamomile prevented inflammation and alveolar bone resorption in animals. - GreenMedInfo Summary
Dry Extract of Matricaria Recutita L. (Chamomile) Prevents Ligature-Induce Alveolar Bone Resorption in Rats via Inhibition of TNF-α and IL-1β.
J Periodontol. 2016 Jan 16:1-17. Epub 2016 Jan 16. PMID: 26777767
Mariana Vasconcelos Guimarães
BACKGROUND: Matricaria recutita L. (chamomile) has demonstrated anti-inflammatory activity. Accordingly, we have evaluated the ability of the Matricaria recutita extract (MRE) to inhibit proinflammatory cytokines and its influence on alveolar bone resorption (ABR) in rats.
METHODS: Wistar rats were subjected to ABR by ligature with nylon thread in the second upper left molar, with contralateral hemiarcade as control. Rats received Tween® 80 (TW80; vehicle) or MRE (10, 30 and 90 mg/kg) 1 h before ligature and daily until the 11(th) day. The periodontium was analyzed by macroscopy, histometry, histopathology and immunohistochemistry for the receptor activator of nuclear factor kappa-B ligand (RANKL), osteoprotegerin (OPG) and tartrate-resistant acid phosphatase (TRAP). The gingival tissue was used to quantify the myeloperoxidase activity (MPO) and tumor necrosis factor-alpha (TNF-α) and interleukin-1β (IL-1β) levels by ELISA. Blood samples were collected to evaluate bone-specific alkaline phosphatase (BALP), leukogram anddosages of aspartate and alanine transaminases (AST/ALT), urea and creatinine. Aspects of liver, kidneys, spleen and body mass variations were also evaluated.
RESULTS: The 11 days of ligature induced bone resorption, low levels of BALP, leukocyte infiltration, increase of MPO, TNF-α and IL-1β, immunostaining increase for RANKL and TRAP, reduction of OPG and leukocytosis, which were significantly prevented by MRE, except for the low levels of BALP and the leukocytosis. Additionally, MRE did not alter organs nor body weights of rats.
CONCLUSION: MRE prevented the inflammation and alveolar bone resorption by reducing TNF-α and IL-1β, preventing the osteoclast activation via the RANKL-OPG axis, without interfering with bone anabolism.