Chebulic acid has cytoprotective effect on tert-butyl hydroperoxide-induced hepatotoxicity in HepG2 cells. - GreenMedInfo Summary

Although chebulic acid isolated fromhas diverse biological effects, its effects on the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and the expression of downstream genes have not been elucidated. The purpose of this research is to investigate the hepatoprotective mechanism of chebulic acid against oxidative stress produced by-butyl hydroperoxide (-BHP) in liver cells. The treatment with chebulic acid attenuated cell death in-BHP-induced HepG2 liver cells and increased intracellular glutathione content, upregulated the activity of heme oxygenase-1, and also increased the translocation of Nrf2 into the nucleus and Nrf2 target gene expression in a dose-dependent manner. The exposure of chebulic acid activated the phosphorylation of mitogen-activated protein kinases. The overall result is that chebulic acid has cytoprotective effect on-BHP-induced hepatotoxicity in HepG2 cells through Nrf2-mediated antioxidant enzymes.