Ameliorates Oxidative Stress and Improves the Muscle Regenerative Capacity of Young and Old Sprague-Dawley Rats.
Nutrients. 2020 Dec 7 ;12(12). Epub 2020 Dec 7. PMID: 33297295
Nurhazirah Zainul Azlan
Muscle atrophy in ageing is a multifactorial degenerative process impacted by cellular ageing biology, which includes oxidative stress.is a coccoid green eukaryotic microalga rich in antioxidants. The aim of this study was to determine the effect ofin ameliorating oxidative stress, thus elucidating its mechanism in improving muscle mass, strength and function in young and old rats. Fifty-six male Sprague-Dawley (SD) rats aged 3 months (young) and 21 months (old) were divided into three groups: Group 1 (control) was given distilled water; Group 2 was treated with 150 mg/kg body weight (BW) ofand Group 3 was treated with 300 mg/kg BW offor three months. Grip and muscle strength and muscle integrity were determined on days 0, 30, 60, and 90 of treatment. Urine and blood were collected on days 0 and 90 of treatment for oxidative stress marker determination, while the gastrocnemius muscles were collected for muscle oxidative stress analysis. Increased grip strength of the front and hind paws was observed in young-treated rats on days 30, 60, and 90 compared to the untreated control on the same days (<0.05). There was a significant increase in lean bone mineral content (BMC) in young rats treated with 300 mg/kg BWcompared to untreated rats on days 30 and 60. The fat mass was significantly decreased in young and old-treated rats on day 90 compared to the untreated control. The total path was significantly increased for old rats treated with 300 mg/kg BWon days 60 and 90 compared to day 0. Young and old-treated rats demonstrated a significant decrease in urinary isoprostane Fand plasma creatine kinase-MM (CKMM) compared to the control on day 90. A significant decrease in malondialdehyde (MDA) and 4-hydroxyalkenal (HAE) levels were observed in young and old rats treated with.improved the muscle mass, strength, and function in young and old rats. This effect could be due to its potency in ameliorating oxidative stress in the skeletal muscle of young and old rats.