Article Publish Status: FREE
Abstract Title:

Improves the Regenerative Capacity of Young and Senescent Myoblasts and Promotes Muscle Regeneration.

Abstract Source:

Oxid Med Cell Longev. 2019 ;2019:3520789. Epub 2019 Jun 4. PMID: 31281573

Abstract Author(s):

Nurhazirah Zainul Azlan, Yasmin Anum Mohd Yusof, Ekram Alias, Suzana Makpol

Article Affiliation:

Nurhazirah Zainul Azlan


Sarcopenia is characterized by the loss of muscle mass, strength, and function with ageing. With increasing life expectancy, greater attention has been given to counteracting the effects of sarcopenia on the growing elderly population., a microscopic, unicellular, green alga with the potential for various pharmaceutical uses, has been widely studied in this context. This study is aimed at determining the effects ofon promoting muscle regeneration by evaluating myoblast regenerative capacity. Human skeletal myoblast cells were cultured and underwent serial passaging into young and senescent phases and were then treated with, followed by the induction of differentiation. The ability ofto promote myoblast differentiation was analysed through cellular morphology, real-time monitoring, cell proliferation, senescence-associated-galactosidase (SA--gal) expression, myogenic differentiation, myogenin expression, and cell cycle profiling. The results obtained showed that senescent myoblasts exhibited an enlarged and flattened morphology, with increased SA--gal expression, reduced myogenic differentiation, decreased expression of myogenin, and an increased percentage of cells in the/phase. Treatment withresulted in decreased SA--gal expression and promotion of myogenic differentiation, as observed via an increased fusion index, maturation index, myotube size, and surface area and an increased percentage of cells that stained positive for myogenin. In conclusion,improves the regenerative capacity of young and senescent myoblasts and promotes myoblast differentiation, indicating its potential to promote muscle regeneration.

Study Type : Human In Vitro

Print Options

Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2022 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.