Citrus aurantium dry extracts ameliorate adipocyte differentiation of 3T3-L1 cells exposed to TNFα by down-regulating miR-155 expression. - GreenMedInfo Summary

L. dry extracts (de) improve adipogenesis in vitro. These effects are dependent from an early modulation of CCAAT/enhancer-binding protein beta () expression and cyclic Adenosine Monophosphate (cAMP) response element-binding protein () activation.andare also targets of. This study investigated whetherde regulatesexpression in the early stages of adipogenesis and whether it ameliorates adipocyte differentiation of cells exposed to tumor necrosis factor-alpha (TNFα). Adipogenic stimuli (AS) were performed in 3T3-L1 pre-adipocytes treated withde, TNFα, or both. Gene and miRNA expression were determined by quantitative real-time PCR. Adipogenesis was evaluated by Oil-Red O staining.de treatment enhanced AS effects during the early adipogenesis phases by further down-regulatingexpression and increasing bothandmRNA and protein levels. At variance, TNFα inhibited 3T3-L1 adipogenesis and abolished AS effects on,, andexpression. However, in cells exposed to TNFα,de improved adipocyte differentiation and restored the AS effects on miRNA and gene expression at early time points. In conclusion, this study identifieddown-regulation as part of the mechanism through whichde enhances adipogenesis of pre-adipocytes in vitro. Furthermore, it provides evidence ofde efficacy against TNFα negative effects on adipogenesis.