Combination of berberine with ciprofloxacin reduce a multi-resistantstrain biofilm formation by depressing mRNA expressions of,and.
J Vet Sci. 2018 Oct 8. Epub 2018 Oct 8. PMID: 30304890
Bacterial biofilms have been demonstrated to be closely related to clinical infections and mainly contribute to drug resistance. Berberine, which is the main component of, has been reported to have efficient antibacterial activity. This study aimed to investigate the potential effect of combination of berberine with ciprofloxacin (CIP) to inhibitbiofilm formation and the effect on the related genes (,,) expressions. The fractional inhibitory concentration index of the combination of berberine with CIP is 0.75 showing a synergistic antibacterial effect. The biofilm adhesion rate and growth curve showed that the multi-resistantstrain had potential ability to form biofilm relative to CVCC528, and the antibiofilm effects were in a dose-dependent manner. Biofilm microstructures were rarely observed at 1/2×MIC/FIC concentrations, and the combination showed stronger antibiofilm effect than each of the antimicrobial agent used alone at 1/4×FIC concentration.,andmRNA expressions were significantly repressed (＜ 0.01) at 1/2×MIC/FIC concentrations, and the combination repressed mRNA expressions more strongly at 1/4×FIC concentration. The results indicated that the combination of berberine and CIP showed synergistic effect and more effective to inhibitbiofilm formation via repression of,andmRNA expressions.