Article Publish Status: FREE
Abstract Title:

Separation and purification and in vitro anti-proliferative activity of leukemia cell K562 of Galium aparine L. petroleum ether phase.

Abstract Source:

Saudi Pharm J. 2016 May ;24(3):241-4. Epub 2016 Apr 24. PMID: 27275108

Abstract Author(s):

Guoqing Shi, Jiang Liu, Wen'en Zhao, Yanqi Liu, Xinfeng Tian

Article Affiliation:

Guoqing Shi


To explore material basis of in vitro anti-proliferative activity of leukemia cell K562 of petroleum ether phase of product resulting from Galium aparine L. 60% ethanol extraction, the experiment adopts column chromatography combined with thin layer preparation, isolates and purifies petroleum ether, conducts structural identification of obtained single compound and applies MTT method for viability assay of in vitro anti-proliferative activity of leukemia cell K562. Experimental results show that G. aparine L. petroleum ether contains mainlyβ-sitosterol, daucosterol and dibutyl phthalate and other substances. Under experimental conditions, the three could inhibit the proliferation of leukemia cell K562 with dose-effect and time-effect relationship, of which dibutyl phthalate has strongest activity. Dibutyl phthalate with excellent activity, β-sitosterol with rich content and moderate effect should be the main contributor to its biological activity.

Study Type : In Vitro Study

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Sayer Ji
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