Abstract Title:

Polyphenol-Rich Cranberry Extracts Modulate Virulence ofBiofilms Implicated in the Pathogenesis of Early Childhood Caries.

Abstract Source:

Pediatr Dent. 2019 Jan 15 ;41(1):56-62. PMID: 30803479

Abstract Author(s):

Nebu Philip, Shaneen J Leishman, Hmhn Bandara, Laurence J Walsh

Article Affiliation:

Nebu Philip


The purpose of this study was to investigate the effects of polyphenol-rich cranberry extracts on dual-speciesbiofilms implicated in contributing to the severity of early childhood caries.biofilms were grown on saliva-coated hydroxyapatite discs (s-HA) mounted on the high-throughput Amsterdam Active Attachment model. The s-HA discs were treated with the cranberry extracts/vehicle control for five minutes just before biofilm growth and subsequently, for similar exposure times, after 12 hours and 24 hours of biofilm growth. The treated 24-hour-old biofilms were then assessed for acidogenicity, metabolic activity, exopolysaccharide (EPS)/microbial biovolumes, structural organization, and colony forming unit (CFU) counts.Treatment with 500 to 1,000μg/mL of the cranberry extracts produced significant reductions in acidogenicity and metabolic activity (P<0.0001) compared to the control-treated biofilms. A significant decrease in biovolumes of the EPS (P=0.003) and microbial biofilm components (P=0.007) was also seen. Qualitative assessment of confocal biofilm images revealed that the cranberry extract disrupted biofilm structural architecture. Finally, significantly fewer S. mutans (P=0.006) and C. albicans (P=0.036) CFUs were recovered from the cranberry-treated biofilms than from the control-treated bio-films. Conclusions: Cranberry extracts inhibited cariogenic virulence properties ofdual-species biofilms in an in vitro model.

Study Type : In Vitro Study

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