Abstract Title:

Utility of six fungicides for selective isolation of Evlachovaea sp. and Tolypocladium cylindrosporum.

Abstract Source:

Mycopathologia. 2009 Jun;167(6):341-50. Epub 2009 Feb 11. PMID: 19205922

Abstract Author(s):

Luiz F N Rocha, Christian Luz

Article Affiliation:

DMIPP, Instituto de Patologia Tropical e Saúde Pública (IPTSP), Universidade Federal de Goiás (UFG), Goiânia, GO, Brazil.


The effects of the fungicides dodine, benomyl, thiabendazole, mancozeb, cupric sulfate, and copper oxychloride were examined in vitro upon germination and further development of Evlachovaea sp. and Tolypocladium cylindrosporum. Fungicidal activity depended on concentrations and varied among products, fungi and the strains tested. Depending on the fungicidal concentration, germination of conidia was induced but germlings produced neither mycelium nor new conidia. There was a good recovery of both Evlachovaea sp. and T. cylindrosporum from previously sterilized soils with fungicide-supplemented medium. Fungi were resistant to copper oxychloride up to 30 g/l, and this fungicide was found to have no utility for a selective medium. Minimal fungicide concentrations for successful isolations were 1 mg/l for benomyl, 200 mg/l for cupric sulfate, 50 mg/l for dodine, 100 mg/l for mancozeb, and 4 mg/l for thiabendazole. Thiabendazole, which is easy to obtain and can be used in low quantities, showed the greatest utility for a selective medium with these entomopathogenic fungi.

Study Type : In Vitro Study
Additional Links
Problem Substances : Cupric Sulfate : CK(54) : AC(0)

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