Curcumin inhibits lipopolysaccharide and lipoteichoic acid-induced expression of proinflammatory cytokines and production of PGEin the primary bubaline endometrial stromal cells.
Mol Biol Rep. 2020 Dec ;47(12):10015-10021. Epub 2020 Nov 10. PMID: 33174083
Infection of the uterus with Gram-positive Trueperella pyogenes and Gram-negative Escherichia coli is a common cause of postpartum endometritis in the cattle and buffalo and the condition is treated with antimicrobial drugs. The presence of drug residues in the milk and development of resistant bacteria necessitate the evaluation of alternate therapies for endometritis. Accordingly, we tested the immunomodulatory effect of curcumin in the bubaline endometrial stromal cells after treatment with the lipoteichoic acid (LTA) of Gram-positive Staphylococcus aureus and lipopolysaccharide (LPS) of Gram-negative E. coli that activate toll-like receptors (TLR-2 and TLR-4, respectively). Confluent primary culture of endometrial stromal cells was treated with LTA (1 µg/mL) and/or LPS (0.1 µg/mL), in the presence or absence of curcumin (30 µM for 24 h). PGEwas assayed in the supernatant and the relative expression of proinflammatory cytokines (PICs) (IL1B, IL6, IL8 and TNFA) transcripts were quantified using real-time PCR. LTA was not effective in stimulating PGEproduction or upregulating the PIC expression except IL8. LTA+LPS increased PGEproduction and upregulated IL6 and IL8 genes. Curcumin inhibited the basal and LTA+LPS induced production of PGEand upregulation of PIC production. It was apparent that LPS, but not LTA, is a potent stimulator of PGEfrom the bubaline endometrial stromal cells. Curcumin downregulated the expression of LPS and/or LTA induced PICs and PGEand may be an alternate to antimicrobial drugs for the therapeutic management of endometritis.