Abstract Title:

Protective effects of curcumin against gamma ray induced conformational change of human serum albumin.

Abstract Source:

Int J Biol Macromol. 2017 Jun ;99:600-607. Epub 2017 Mar 6. PMID: 28274864

Abstract Author(s):

Turban Kar, Pijush Basak, Rittik Kumar Ghosh, Maitree Bhattacharyya

Article Affiliation:

Turban Kar


This study explores the possibility for protection by curcumin during the molecular and structural changes of human serum albumin (HSA) exposed to gamma irradiation. We used a combination of spectroscopic methods to probe the conformational ensemble of the irradiated HSA and finally evaluated the extent of restoration by curcumin. SDS-PAGE study unfolded the formation of cross linked aggregates as a consequence of increasing exposure of gamma radiation. CD and FTIR spectroscopy indicated significant decrease in the alpha helix content of HSA from 57% to 15% with increasing radiation doses. Steady state and time resolved fluorescence studies complemented the spectroscopic measurements, when lifetime decay was significantly reduced from 6.35ns to 0.37ns. Hydrophobic study showed the effectiveness of curcumin for protection at low dose of gamma irradiated HSA samples. We integrated these observations to investigate protein aggregation under increasing gamma radiation and estimated the same in presence of curcumin. It was elucidated, that when HSA is irradiated at low dose of gamma radiation in presence of curcumin, it is capable of retaining the characteristic properties to a higher extent indicating stabilization of molecular structure of HSA by curcumin. A model for curcumin based protection has been proposed utilizing ThT assay.

Print Options

Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2022 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.