Curcumin protects against staurosporine toxicity in rat neurons. - GreenMedInfo Summary
Curcumin protects against staurosporine toxicity in rat neurons.
Neurosci Bull. 2012 Oct ;28(5):606-10. Epub 2012 Oct 3. PMID: 23054638
State Key Laboratory of Biomembrane and Membrane Biotechnolog, College of Life Sciences, Peking University, Beijing, 100871, China.
OBJECTIVE: Curcumin is extracted from the turmeric plant (Curcuma longa Linn.) and is widely used as a food additive and traditional medicine. The present study investigated the activity of curcumin against staurosporine (STS) toxicity in cell culture.
METHODS: Rat hippocampal neurons in primary culture were exposed to STS (20μmol/L) and treated with curcumin (20 μmol/L). Cell viability was tested by MTT assay and reactive oxygen species (ROS) were measured using the MitoSOX™ red mitochondrial superoxide indicator. Western blot was used to assess changes in the levels of caspase-3 (Csp3), heat shock protein 70 (Hsp70) and Akt.
RESULTS: The results showed that curcumin protects against STS-induced cytotoxicity in rat hippocampal neurons. Csp3, Hsp70, Akt and ROS activation may be involved in this protection.
CONCLUSION: Curcumin could be a potential drug for combination with STS in cancer treatment to reduce the unwanted cytotoxicity of STS.