Abstract Title:

Curcumin Targets Circulating Cancer Stem Cells by Inhibiting Self-Renewal Efficacy in Non-Small Cell Lung Carcinoma.

Abstract Source:

Anticancer Agents Med Chem. 2016 Sep 23. Epub 2016 Sep 23. PMID: 27671306

Abstract Author(s):

Sheefa Mirza, Aakanksha Vasaiya, Hemangini Vora, Nayan Jain, Rakesh Rawal

Article Affiliation:

Sheefa Mirza

Abstract:

BACKGROUND: The ultimate goal of the study was to find a role of curcumin in targeting lung cancer stem cells by reducing their self-renewal efficiency causing DNA damage.

MATERIALS AND METHODS: Circulating lung cancer stem cells were isolated by sphere formation assay and further analysed by flow-cytometry and qRT-PCR for the presence of stem cell and stem cell transcription markers. IC50 values of gemcitabine and curcumin was analysed by MTT assay, while curcumin induced DNA damage was scrutinized by single cell gel electrophoresis assay.

RESULTS AND CONCLUSION: Our results demonstrated that curcumin significantly affect the self-renewal ability of circulating lung cancer stem cells. The no. of spheres formed in the presence of curcumin was shown to be significantly decreased. Additionally, our results depicted that 4.52±0.72 % and 95.47±0.72 % (p< 0.0001) of DNA material was found to be present in head and tail respectively, suggesting curcumin's functional potential to cause DNA damage. Thus, we can conclude that curcumin can be used to target lung cancer stem cells which is responsible for the disease progression and metastasis by causing DNA damage or inhibiting their DNA repair mechanisms.

Study Type : In Vitro Study

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