Curcumol attenuates epithelial-mesenchymal transition of nasopharyngeal carcinoma cells via TGF-β1.
Mol Med Rep. 2018 Jun ;17(6):7513-7520. Epub 2018 Mar 28. PMID: 29620189
The current study aimed to identify the effect and primary mechanism of Curcumol on the migration of nasopharyngeal carcinoma (NPC) cells in vitro and in vivo. Curcumol was dissolved in absolute ethyl alcohol and the experiment was performed in NPC 5‑8F cells in vitro and in vivo. The effect of different concentrations of Curcumol on cell migration was determined using wound healing and Transwell assays. A cell counting kit‑8 (CCK‑8) assay was also performed in order to determine cell viability. Flow cytometry was used to detect the effect of Curcumol on apoptosis. The expression of epithelial‑mesenchymal transition (EMT)‑associated proteins and genes was evaluated by western blotting, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and ELISA. In addition, the antitumor activity of Curcumol was investigated in female BALB/C nude mice with orthotopic tumor implants. The results indicated that cell apoptosis was increased and the viability of NPC 5‑8F cells was decreased following treatment with Curcumol at doses of 0.1, 0.2 and 0.4 µM/ml. The results of in vivo experiments indicated that tumor growth and weight were decreased following Curcumol administration. Furthermore, the results of western blotting and RT‑qPCR demonstrated that Curcumol altered the level of E‑cadherin and N‑cadherin in a dose‑dependent manner in vivo. Curcumol also regulated the secretion of protein markers in the serum that were associated with EMT and TGF‑β1 in the 5‑8F xenograft mouse model. Thus, the results indicated that Curcumol induced TGF‑β1‑mediated EMT arrest by regulating E‑cadherin and N‑cadherin, which may prevent further development of NPC.