Effect of Curcumol on the Fenestrae of Liver Sinusoidal Endothelial Cells Based on NF-B Signaling Pathway.
Evid Based Complement Alternat Med. 2020 ;2020:8590638. Epub 2020 May 28. PMID: 32595742
Objective: To study the effect of curcumol on liver sinusoidal endothelial cells (LSECs) and to analyze the mechanism of antihepatic fibrosis.
Methods: The effects of drug intervention on cell proliferation rates were detected by MTT assay. The expression of NF-B was detected by RT-PCR and WB. The NF-B expression and entry into the nucleus were detected by immunofluorescence; scanning electron microscopy was used to observe the changes of LSECs fenestrae.
Results: MTT results showed that the interference of cell proliferation in each group was small. RT-PCR showed that the expression of NF-B in the curcumol intervention group was significantly lower than that in the positive control group (<0.05). The WB detection found that, in the curcumol intervention group, the expression of pNF-B in the NF-B signaling pathway was significantly lower than that in the positive control group (<0.05). Scanning electron microscopy showed that the LSEC fenestrae were significantly improved compared with the positive control group.
Conclusion: Curcumol may be one of the mechanisms of antihepatic fibrosis by inhibiting the activity of the NF-B signaling pathway and increasing the fenestrae of LSECs.