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Article Publish Status: FREE
Abstract Title:

Cytotoxic potentials of silibinin assisted silver nanoparticles on human colorectal HT-29 cancer cells.

Abstract Source:

Bioinformation. 2020 ;16(11):817-827. Epub 2020 Nov 30. PMID: 34803254

Abstract Author(s):

Kiren Jackson, Ezhilarasan Devaraj, Thangavelu Lakshmi, Shanmugam Rajeshkumar, Kamal Dua, Dinesh Kumar Chellappan, Subramanian Raghunandhakumar

Article Affiliation:

Kiren Jackson

Abstract:

It is of interest to study the cytotoxicity of silibinin assisted silver nanoparticles in human colorectal (HT-29) cancer cells. Silver nanoparticles were synthesized using silibinin as a reducing agent. The synthesized silibinin assisted silver nanoparticles ( SSNPs) were characterized and analyzed using a transmission electron microscope and spectrophotometer. The SSNPs synthesized in this study are spherical and their size ranges from 10 to 80 nm. HT-29 cells were treated with different concentrations (2, 4, 6, 8 and 10 ng/mL) of SSNPs and cytotoxicity was evaluated. The apoptosis was using flow cytometry. p53 protein expression using western blot. SSNPs are induced a decrease in viability and increased concentration-dependent cytotoxicity in HT-29 cells. SSNPs treatment also caused apoptosis-related morphological changes. SSNPs treatments at 8 and 16 ng/ml showed a prominent apoptotic change i.e., 70.3% and 83.6% respectively, and decreased viability of HT-29 cells 20% and 11.2% respectively as compared to control cells. SSNPs treatments induced p53 expression in HT-29 cells. Data shows that SSNPs have the potential to induce apoptosis in colorectal cancer cells. This provides insights for the further evaluation of SSNPs in fighting colon cancer.

Study Type : In Vitro Study

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