The effect of caffeic acid phenethyl ester on cell cycle control gene expressions in breast cancer cells. - GreenMedInfo Summary

We aimed to find the effect of caffeic acid phenethyl ester (CAPE) on the expression profiles of cell cycle control genes in breast cancer cell line (MCF-7). The cytotoxic effect of CAPE on MCF-7 cell line was found with an XTT analysis. Total RNA was isolated from the cells exposed to ICdose and untreated control cells. Expressions of genes related to cell cycle control (,,,,) were evaluated by qRT-PCR by the LightCycler 480 System (Roche).andhousekeeping genes were used for the normalization of gene expressions. ICvalue of CAPE in MCF-7 cells was calculated as 75µM. It was shown that ICdose of CAPE induced significant upregulation in expressions of cell cycle control genes, compared to control cells. CAPE increases the expression of genes that are important in cell cycle control, suggesting that this component can be used as an effective chemopreventive agent in breast cancer cells.