Effect of electroacupuncture on expression of NF-κB and TNF-α in renal tissue of rats with acute cerebral infarction. - GreenMedInfo Summary
[Effect of electroacupuncture on expression of NF-κB and TNF-α in renal tissue of rats with acute cerebral infarction].
Zhen Ci Yan Jiu. 2021 Dec 25 ;46(12):1005-10. PMID: 34970876
Jun-Chuan Zhang
OBJECTIVE: To observe the effect of electroacupuncture (EA) on motor function, serum Cystatin C (Cys C) content, and expressions of tumour necrosis factor-α (TNF-α) and nuclear factor-kappa B (NF-κB) in renal tissue of rats with acute cerebral infarction (ACI), so as to explore its underlying mechanisms in protecting renal tissue after ACI.
METHODS: Seventy-two male SD rats were randomly divided into three groups: sham operation, model and EA groups which were further randomly allocated to 1 d, 3 d, 7 d and 14 d subgroups (=6 per subgroup). The ACI model was established by occlusion of the middle cerebral artery (MCAO). Rats of the EA group received EA of "Neiguan" (PC6) and "Zusanli" (ST36) for 30 min, once daily for 1, 3, 7 and 14 days, respectively. The motor function and content of Cys C were determined on the 1, 3, 7and 14day after ACI. The expressions of TNF-α and NF-κB were detected by immunohistochemistry.
RESULTS: Compared with the sham operation group, the motor function scores and the content of Cys C increased significantly on the 1, 3, 7and 14d (<0.01), while the numbers of TNF-α and NF-κB positive cells of the model group increased significantly on the 3, 7and 14d (<0.01). After EA treatment, the motor function scores and the content of Cys C on the 7, and 14d, and the numbers of TNF-α and NF-κB positive cells on the 3, 7and 14d obviously decreased (<0.05).
CONCLUSION: EA at PC6 and ST36 can improve motor function and alleviate renal injury in ACI rats, possibly by regulating the expression of TNF-α, NF-κB in renal tissue and Cys C in serum.
