The Effect ofon Th1/Th2 and Th17/T Regulatory in a Mouse Model of Allergic Asthma.
Front Pharmacol. 2017 ;8:458. Epub 2017 Aug 7. PMID: 28824424
Asthma is a chronic inflammatory disease with no definite treatment and more research is needed to overcome this condition. The aim of this study was to investigate the effect of the extract of() as a medicinal plant on cytokine genes expression in an experimental mouse model of asthma. Adult mice were randomly divided into the following groups: control (C), untreated asthma (A), asthmatic groups treated with dexamethasone (D) andextract (200, 400, and 800μg/mL; Z1, Z2, and Z3, respectively), (for groups C, A, and D= 5 and for groups Z1, Z2, and Z3= 6). For induction of the mouse model of asthma, animals were sensitized with intraperitoneal injection and inhalation of ovalbumin (OVA). The number of T helper (Th) subtype cells (using flow cytometry) and the levels of IFN-γ, FOXP3, IL-4, TGF-β, IL-17 gene expression (by real time PCR) were assessed in mice splenocytes. The observed changes in spleen cells of group A compared to group C were increased number of Thand Thcells, enhancement of gene expression of IL-4, IL-17, and TGF-β (<0.001 for all cases), reduction of Thcells and Th/Thratio (<0.001 for both cases) and decrease in gene expression of IFN-γ, FOXPand IFN-γ/IL-4 ratio (<0.01 for IFN-γ and<0.001 for other cases). The observed changes in spleen cells of treated compared to untreated A group were enhancement of Treg cells and Th/Thratio (<0.001 for both cases), increase in IFN-γ (<0.05) and FOXP(<0.001) gene expression and IFN-γ/IL-4 ratio (<0.01) as well as reduction of Thand Thcells (<0.01 to<0.001), decrease gene expression of IL-4, IL-17, and TGF-β (<0.05 to<0.001). The findings showed that the extract ofdecreased pro-inflammatory cytokines in asthma (IL-4 and IL-17 and TGF-β) but increased anti-inflammatory cytokines (IFN-γ) gene expression and the number of Treg (FOXP3) in splenocytes of asthmatic mice which may indicate the specific therapeutic effect of the plant extract in allergy, autoimmunity, and infectious diseases via potentiating Thand suppressing Th2 and Thcells.