Abstract Title:

[Effects of electroacupuncture on motor function and calpain expression in rats with acute spinal cord injury].

Abstract Source:

Zhen Ci Yan Jiu. 2020 Dec 25 ;45(12):968-72. PMID: 33415854

Abstract Author(s):

Jing Liu, Ming-Zhong Xiao

Article Affiliation:

Jing Liu


OBJECTIVE: To observe the effect of electroacupuncture (EA) on motor function, calpain and calpastatin expression in rats with spinal cord injury, so as to explore the mechanism of EA underlying improvement of acute spinal cord injury.

METHODS: Thirty male SD rats were randomly divided into sham operation group, model group and EA group, with 10 rats in each group. The acute moderate spinal cord injury model was established by using a NYU spinal cord impactor. EA was applied to"Jizhong"(GV6) and"Mingmen"(GV4) for 30 min, once daily for 28 d. The Basso, Beattie and Bresnahan (BBB) rating scale (0 to 21 points) was used to assess changes of locomotor function. Histopathological changes of the injured spinal cord were observed after sectioning and Nissl staining, and the expression levels of calpain1, calpain2 and calpastatin mRNA and protein in the spinal cord tissues were detected by using quantitative real-time PCR and Western blot, respectively.

RESULTS: The BBB score of the model group was significantly lower than that of the sham operation group (<0.01), and was significantly higher in the EA group than that of the model group on 14th and 28th day (<0.01). Compared with the sham operation group, the number of neurons in the model group decreased, and Nissl body stained cells decreased or even disappeared, which was evidently milder in the EA group. Compared with the sham operation group, the expression levels of calpain1 mRNA and protein in the spinal cord of the model group were significantly increased (<0.01), while the expression levels of calpastatin mRNA and protein were significantly reduced (<0.01). Following EA intervention, in contrast to the model group, the expression levels of calpain1 mRNA and protein in the EA group were significant down-regulated (<0.01), calpastatin mRNA and protein expression levels were significantly up-regulated(<0.01). There was no significant difference in calpain2 mRNA and protein expression among the 3 groups(>0.05).

CONCLUSION: EA can improve the locomotor function of rats with spinal cord injury, which may be related to its effect in inhibiting the activity of calpain in the injured spinal cord.

Study Type : Animal Study

Print Options

Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2022 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.