Effects of exercise and stevia on renal ischemia/reperfusion injury in rats. - GreenMedInfo Summary
Effects of exercise and stevia on renal ischemia/reperfusion injury in rats.
Acta Sci Pol Technol Aliment. 2019 Jul-Sep;18(3):317-332. PMID: 31569913
Fathy H Elsaid
BACKGROUND: The present work was designed to study the effects of methanolic stevia extracts and aerobic exercise and combination of both on renal I/R injury in male rats.
METHODS: 60 adult male Sprague-Dawley rats were subdivided into five equal groups as sham, control, exercise, stevia, and stevia plus exercise group. After 5 weeks of exercise and stevia, animals were exposed to 45 min of left renal ischemia and right nephrectomy followed by reperfusion. Serum creatinine, creatinine clearance, fractional Na excretion (FENa+), malondialdehyde (MDA), reduced glutathione (GSH) and catalase (CAT) levels in kidney tissues were measured. Also, renal histopathology and the expression of caspase-3 by immunohistochemical examination were done.
RESULTS: The results showed that stevia, exercise or combination of stevia and exercise caused a significant decrease in serum level of creatinine (p<0.001) and FENa+ (p<0.001) and an increase in creatinine clearance (p<0.001). Moreover, this caused a significant decrease in (MDA; p<0.046) and an increase in GSH (p<0.01) and CAT (p<0.01), as well as causing a significant decrease in caspase 3 expression compared to the control group.
CONCLUSIONS: Pretreatment with either stevia or exercise of combination of both seem to have protective effects on renal I/R injury. However, the protective effect of exercise against renal I/R injury seems to be less than stevia. These effects might be due to attenuation of oxidative stress and apoptosis in kidney tissues.