Effects of Inonotus obliquus polysaccharides on proliferation, invasion, migration, and apoptosis of osteosarcoma cells. - GreenMedInfo Summary

Objectives: To observe the effect ofpolysaccharide (IOP) on the proliferation, invasion, migration, and apoptosis of osteosarcoma cells and to elucidate its underlying molecular mechanism.

Methods: IOP was extracted from, human osteosarcoma MG-63 cells and U2OS cells were cultured in vitro, and the effects of IOP on the proliferation, migration, invasion, and apoptosis of MG-63 cells and U2OS cells were determined by CCK-8 assays, cell scratch assays, transwell assays, and flow cytometry, respectively. Western blot was used to detect the expression of related proteins in the Akt/mTOR and NF-B signaling pathways.

Results: Compared with the control group, MG-63 cells and U2OS cells treated with IOP of 80 g/ml, 160 g/ml, and 320  g/ml in the experimental group had significantly lower proliferation activity, decreased migration and invasion ability, and increased apoptosis rate (<0.05). Furthermore, IOP could significantly inhibit the activation of the Akt/mTOR and NF-B signaling pathway (<0.05).

Conclusion: IOP can regulate the proliferation, migration, invasion, and apoptosis of osteosarcoma cells by inhibiting the activation of the Akt/mTOR signaling pathway. It has antitumor activity on osteosarcoma and has the potential of clinical application in osteosarcoma treatment.