Abstract Title:

[Effect of epigallocatechin-3-gallate on the proliferation and osteogenesis of human periodontal ligament cells].

Abstract Source:

Zhonghua Kou Qiang Yi Xue Za Zhi. 2016 Dec 9 ;51(12):758-764. PMID: 27978918

Abstract Author(s):

J Liu, Y Lu, Z Z Wang, W Z Du, D D Pei

Article Affiliation:

J Liu


Objective: To evaluate the effect of epigallocatechin-3-gallate (EGCG) treatment on the proliferation and osteogenic differentiation of human periodontal ligament cell (hPDLC) and to explore the potential role of EGCG in promoting periodontal hard tissue regeneration. Methods: The hPDLC was isolated from periodontal ligament tissue obtained from freshly extracted human teeth. The effect of treatments with various concentrations of EGCG (0μmol/L, 2 μmol/L, 4 μmol/L, 6 μmol/L, 8 μmol/L and 10 μmol/L) on cell proliferations were determined by cell counting kits (CCK) after 24-, 48- and 72-hour-incubations, respectively. Osteogenic differentiation abilities of hPDLCs were assessed by using alkaline phosphatase (ALP) activity testsafter 7- and 14-day-incubations, respectively. The mineralized nodules were quantitatively examined and analyzed by using alizarin red staining after 21-day-incubation. The real-time PCR (RT-PCR) assays were conducted fordetecting the expressions of Runt related transcription factor-2 (Runx2), ALPand collagen type Ⅰ (COL Ⅰ) after 7-day-incubation. Results: Treatment with 4 μmol/L EGCG increased hDPLC proliferation at 24 h, while 8 μmol/L or 10 μmol/L EGCG treatment groups showed inhibiting effects at 24 h and 72 h, respectively. Findings of alizarin redstaining showed orange to red colored extracellular mineralized nodules in all groups. The the A values of 2, 4, 6, 8, 10 μmol/L EGCG groups were 0.119±0.001, 0.167±0.003, 0.173±0.003, 0.110±0.001 and 0.083±0.003, respectively. A values of 2-8 μmol/L EGCG groups were significantly higher than that of the control group, however there was no significant difference of the A values between10 μmol/L EGCG group and the control group (0.077±0.001). Treatments with 2-10 μmol/L EGCG could significantly increase the mRNA expressions of COL Ⅰ and ALP with the highest values in 4-6 μmol/L EGCG treatment groups. Although treatments with 4 and 6 μmol/L EGCG both could increase the mRNA expressions of Runx2, the result in 4 μmol/L group was much better than that of 6 μmol/L group. Conclusions: Treatment of 4 μmol/L EGCG could promote hPDLC proliferation at early stageand treatments with 4-6 μmol/L EGCG could significantly promote the osteogenesis of hPDLCs which might play a promising role in periodontal hard tissue regeneration.

Study Type : In Vitro Study

Print Options

Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2022 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.