Abstract Title:

Epigallocatechin gallate potentially abrogates fluoride induced lung oxidative stress, inflammation via Nrf2/Keap1 signaling pathway in rats: An in-vivo and in-silico study.

Abstract Source:

Int Immunopharmacol. 2016 Jul 26 ;39:128-139. Epub 2016 Jul 26. PMID: 27472294

Abstract Author(s):

Thangapandiyan Shanmugam, Miltonprabu Selvaraj, Senthilraja Poomalai

Article Affiliation:

Thangapandiyan Shanmugam


BACKGROUND: Since this Nrf2-dependent cellular defense response is able to protect multi-organs, including cancer, neurodegenerative diseases, cardiovascular diseases, inflammation and chronic lung injury. The antioxidant and anti-inflammatory potential of Epigallocatechin gallate (EGCG) and Nrf2/Keap1 signaling mechanisms in pulmonary toxicity have not been clarified. In the present study, we demonstrated that protective efficacy of EGCG against fluoride (Fl) induced oxidative stress mediated lung injury in rats.

METHODS: The animals were divided in to four groups. Group 1: Control rats received normal saline; Group 2 rats received EGCG (40mg/kg/bw) alone for four weeks; Group 3 rats received Fl (25mg/kg/bw) alone for four weeks, Group 4 rats received EGCG (90min before administration) along with Fl for four weeks.

RESULTS: Oral administration of Fl (25mg/kg/bw) significantly (p<0.05) increased the ROS, inflammatory cytokines, lung edema, melonaldehyde (MDA) and myeloperoxidase (MPO) in rats. In addition, upon administration of Fl significantly (p<0.05) decreased the antioxidant status, Nrf2, and HO-1 with increased Keap1 protein. Histological and immunohistochemical (iNOS) study also revealed the Fl induced significant (p<0.05) changes in the lung tissue of rats. Pre-administration of EGCG significantly (p<0.05) improved the antioxidant status, and inhibited the oxidative stress, inflammatory cytokines, and Keap1 protein via the activation of Nrf2 translocation in to the nucleus. Moreover, the molecular docking studies also support the antioxidant potential of EGCG and Nrf2 activation.

CONCLUSION: Taken together, our data indicate that EGCG potentially abrogates Fl induced oxidative lung injury by activation of the Nrf2/Keap1 pathway in rats.

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Sayer Ji
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