Article Publish Status: FREE
Abstract Title:

Antiproliferative and Apoptotic Potential of Cyanidin-Based Anthocyanins on Melanoma Cells.

Abstract Source:

Int J Mol Sci. 2017 Apr 30 ;18(5). Epub 2017 Apr 30. PMID: 28468289

Abstract Author(s):

Dumitriţa Rugină, Daniela Hanganu, Zoriţa Diaconeasa, Flaviu Tăbăran, Cristina Coman, Loredana Leopold, Andrea Bunea, Adela Pintea

Article Affiliation:

Dumitriţa Rugină


Elderberries are known for their high anthocyanins content, which have been shown to possess anti-proliferative and anti-cancer effects. Anthocyanins enriched extract (AEE) was obtained from elderberries and was characterized by LC/DAD/ESI-MS analysis. Five cyanidin-based anthocyanins were identified, among which Cy-3-O-samb was the major compound (51%). The total anthocyanins content of AEE was 495 mg Cy-3-O-samb/100 g FW. AEE inhibited proliferation of metastatic B16-F10 murine melanoma cells, in a concentration-dependent manner, with an IC50 of 264.3μg/mL. LDH (lactate dehydrogenase), as a marker of membrane integrity, increased 74% in B16-F10 cells treated with 250 μg/mL AEE, compared to control. It was observed that apoptosis is the mechanism of melanoma cell death after AEE treatment, confirmed morphologically by acridine orange/ethidium bromide double staining and TUNEL analysis. These results indicate that elderberry-derived anthocyanins might be utilized in future applications as topical adjuvant in skin cancer therapy.

Study Type : In Vitro Study

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Sayer Ji
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