Endoplasmic reticulum stress contributes to genistein-induced apoptosis in cervical cancer cells. - GreenMedInfo Summary

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Abstract Title:

Genistein-induced apoptosis is mediated by endoplasmic reticulum stress in cervical cancer cells.

Abstract Source:

Eur Rev Med Pharmacol Sci. 2016 Jul ;20(15):3292-6. PMID: 27467006

Abstract Author(s):

Y-M Yang, Y Yang, W-W Dai, X-M Li, J-Q Ma, L-P Tang

Article Affiliation:

Y-M Yang

Abstract:

OBJECTIVE: Genistein, a major isoflavone found in soybeans, exhibits anti-cancer activity. Endoplasmic reticulum (ER) stress is known to be implicated in apoptosis induced by anti-cancer drugs. This study aimed to characterize the role of ER stress in genistein-induced apoptosis in cervical cancer.

MATERIALS AND METHODS: HeLa cells were treated with genistein or/and 4-phenylbutyric acid. Cell viability and apoptosis were evaluated by MTT assay and flow cytometry. Protein levels were detected by Western blot analysis.

RESULTS: Genistein suppressed the viability of HeLa cells in a dose dependent manner. In addition, genistein caused apoptosis in HeLa cells in a dose dependent manner. Genistein triggered ER stress in HeLa cells, as indicated by the upregulation of glucose-regulated protein 78 (GRP78) and CHOP expression. Furthermore, ER stress inhibitor 4-phenylbutyric acid alleviated genistein-induced apoptosis and ER stress in HeLa cells.

CONCLUSIONS: Our results suggest that ER stress contributes to genistein-induced apoptosis in cervical cancer cells, and genistein is a promising agent for cervical cancer therapy.

Article Published Date : Jun 30, 2016

Study Type : In Vitro Study

Additional Links

Substances : Genistein : CK(931) : AC(430)

Diseases : Cervical Cancer : CK(945) : AC(568)

Pharmacological Actions : Antiproliferative : CK(6801) : AC(6958), Apoptotic : CK(6986) : AC(6931)

Additional Keywords : Dose Response : CK(1769) : AC(699)