Abstract Title:

[Mechanism of epimedium flavonoids in regulating immuno-senescence via nuclear factor-kappa B related signal transduction pathway].

Abstract Source:

Zhongguo Zhong Xi Yi Jie He Za Zhi. 2006 Jul;26(7):620-4. PMID: 16983917

Abstract Author(s):

Xiao-yu Liu, Zi-yin Shen, Jian-hua Huang

Article Affiliation:

Institute of Integrated Traditional Chinese and Western Medicine, Traditional Chinese and Western Medicine, Huashan Hospital, Fudan University, Shanghai.


OBJECTIVE: To explore the mechanism of epimedium flavonoids (EF) in regulating immunosenescence via nuclear factor-kappa B (NF-kappaB) related signal transduction pathway.

METHODS: (1) The apoptosis index (AI) of splenic lymphocyte in aged rats was monitored by flow cytometry, that of young rats was taken as control. (2) The differential expression profile of NF-kappaB related signals in aged rats allocated in the control group (aged rats, group A), the EF treated group (group B), the PDTC (a NF-kappaB inhibitor) treated group (group C) and the PDTC plus EF treated group (group D), was determined and the main significant molecules in them were analyzed with gene microarray of 96 genes related to NF-kappaB signal pathway.

RESULTS: Excessive apoptosis of T lymphocyte cell was seen in aged rats, and it was significantly suppressed in group B and D. In group B, 73 genes were up-regulated to different extent, including 10 of the NF-kappaB/Rel/IB gene family, transduction signal molecule member of NIK/IKK/I B/Rel/NF-kappaB, NF-kappaB regulatory target genes, trans-membrane receptors, transcription factors, and receptor protein, etc. But the up-regulation on NF-kappaB gene family could not be seen in group C and that on others were also alleviated, while in the group D, the NF-kappaB gene family and its related transduction pathway were still activated to some extent. The NF-kappaB gene family showed a markedly common feature after EF intervention, either used alone or in combination with PDTC, i.e. the significant upregulated NF-kappaB1, NF-kappaB2, Rel B and I Bepsilon, and activated NIK/IKK/I B/Rel/NF-kappaB pathway.

CONCLUSION: EF can suppress the excessive apoptosis of splenic lymphocyte in aged rats and activate Rel/NF-kappaB/ I B/IKK and their signal transduction pathway to up-regulate NF-kappaB through adjusting I Bepsilon and I Balpha, which may be the essential mechanism of EF in rebuilding the immune homeostasis of T lymphocyte apoptosis and retarding immunosenescence.

Study Type : Animal Study

Print Options

Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2023 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.