Malacca leaf ethanolic extract () as a hepatoprotector of the liver of mice () infected with.
Vet World. 2020 Jul ;13(7):1457-1461. Epub 2020 Jul 27. PMID: 32848324
Background and Aim: Although existing research confirms the antiparasitic effect of the Malacca plant against, its effect on the liver, one of the target organs ofhas not been investigated. Therefore, this study was conducted to explore the potential of the ethanolic extract of Malacca () leaves in preventing liver damage in mice () caused byinfection.
Materials and Methods: This study was conducted using the livers of 18 mice fixed in 10% neutral-buffered formalin. A completely randomized design with a unidirectional pattern comprising six treatments was used in this study, with each treatment consisting of three replications. Treatment 0 was the negative control group infected with, treatment 1 was the positive control group infected withfollowed by chloroquine administration at a dose of 5 mg/kg BW, and treatments 2, 3, 4, and 5 were groups infected withand administered Malacca leaf ethanolic extracts at doses of 100, 300, 600, and 1200 mg/kg BW, respectively. The extracts were administered orally using a gastric tube for 4 consecutive days. Mice were sacrificed on the 7day and livers were collected for histopathological examination.
Results: Histopathological examination of the livers of mice infected withdemonstrated the presence of hemosiderin, hydropic degeneration, fat degeneration, necrosis, and megalocytosis. However, all these histopathological changes were reduced in the livers of-infected mice treated with various doses of Malacca leaf ethanolic extract. The differences between the treatments were found be statistically significant (p<0.05).
Conclusion: Ethanolic extract of Malacca leaves has the potential to protect against liver damage in mice infected with. The dose of 600 mg/kg BW was found to be the most effective compared with the doses of 100, 300, and 1200 mg/kg BW.