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Article Publish Status: FREE
Abstract Title:

Exosomes cloak the virion to transmit Enterovirus 71 non-lytically.

Abstract Source:

Virulence. 2020 Dec ;11(1):32-38. PMID: 31885311

Abstract Author(s):

Jiaqi Gu, Jing Wu, Daihua Fang, Yang Qiu, Xinran Zou, Xiaonan Jia, Yiqian Yin, Li Shen, Lingxiang Mao

Article Affiliation:

Jiaqi Gu

Abstract:

Enterovirus 71 (EV71) is a non-enveloped virus and it can be released from host cells through a traditional cytolytic manner. Now, we showed EV71 could be spread non-lytically between cells during early viral infection. In order to explain this phenomenon, we separated supernatant fluids of rhabdomyosarcoma (RD) cells cultures infected with EV71 by isopycnic gradient centrifugation. Two populations of virus particles were morphology indistinguishable by transmission electron microscope (TEM). It showed that some EV71 particles were wrapped inside extracellular vesicles which were verified to be exosomes by immunoassay and morphologic analysis. In addition, exosomes containing viral RNA were shed in plasma of EV71-infected encephalitis in children. Our findings indicate that the"non-enveloped" EV71 virions could be wrapped within exosomes which promote their spread in the absence of cell lysis.: EV71: enterovirus 71; EXO: exosome; RD: rhabdomyosarcoma; TEM: transmission electron microscope; HFMD: hand, foot, and mouth disease; HIV: immunodeficiency virus; HCV: hepatitis C virus; HTLV: Human T-cell lymphotropic virus; HAV: hepatitis A virus; MOI: multiplicity of infection; EVs: extracellular vesicles; VP1: viral capsid protein 1; NTA: nanoparticle tracking analysis; CNS: central nervous system.

Study Type : In Vitro Study
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Additional Keywords : Exosomes : CK(35) : AC(10)

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