Abstract Title:

Exosomes with miR-574 transfer anti-HBV activity mediated by the interferon from macrophage to HBV-infected hepatocyte.

Abstract Source:

J Infect Dis. 2020 Jul 15. Epub 2020 Jul 15. PMID: 32663850

Abstract Author(s):

Wenyu Wu, Di Wu, Weiming Yan, Yongli Wang, Jie You, Xiaoyang Wan, Dong Xi, Xiaoping Luo, Meifang Han, Qin Ning

Article Affiliation:

Wenyu Wu


BACKGROUND: Interferon alpha (IFN-α) has been proven effective in treating chronic hepatitis B (CHB) due to its capability to suppress hepatitis B surface antigen (HBsAg) and hepatitis B virus (HBV) covalently closed circular DNA (cccDNA). However, the underlying mechanisms are blurred.

METHODS: We investigated the antiviral activities of exosomes from responders and non-responders to pegylated IFN-α (PegIFN-α) as well as the supernatants of IFN-α-treated THP-1 (the human leukemia monocyte cell line) derived macrophages. Then the expression profiles of exosomal microRNAs (miRNAs) were analyzed using miRNA sequencing. The luciferase reporter assay was used to locate the binding position of HBV genomic sequence targeted by the identified miRNA.

RESULTS: Exosomes from PegIFN-α treated patients particularly responders as well as the supernatants of IFN-α-treated macrophages exhibited anti-HBV activities as manifested by the suppression of HBsAg, hepatitis B e antigen (HBeAg), HBV DNA and cccDNA levels in HBV related cell lines. PegIFN-α treatment upregulated exosomalhsa-miR-193a-5p, hsa-miR-25-5p, and hsa-miR-574-5p that could partially inhibit HBV replication and transcription. Hsa-miR-574-5p reduced pgRNA and polymerase mRNA levels by binding to the 2750-2757 position of HBV genomic sequence.

CONCLUSIONS: Exosomes can transfer IFN-α-related miRNAs from macrophages to HBV-infected hepatocytes, and exhibit antiviral activities against HBV replication and expression.

Study Type : In Vitro Study
Additional Links
Additional Keywords : Exosomes : CK(312) : AC(199)

Print Options

Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2022 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.