Experimental study of the killing effects of oxymatrine on human colon cancer cell line SW1116. - GreenMedInfo Summary

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Abstract Title:

Experimental study of the killing effects of oxymatrine on human colon cancer cell line SW1116.

Abstract Source:

Chin J Dig Dis. 2005 ;6(1):15-20. PMID: 15667553

Abstract Author(s):

Jian Zou, Zhi Hua Ran, Qi Xu, Shu Dong Xiao

Article Affiliation:

Jian Zou

Abstract:

OBJECTIVE: To study the killing effects of oxymatrine (OM) on a human colon cancer cell line, SW1116, and evaluate its antineoplastic mechanism.

METHODS: Methyl thiazolyl tetrazolium (MTT) analysis, flow cytometry, polymerase chain reaction (PCR)-enzyme linked immunosorbent assay (ELISA) and RT-PCR methods were used respectively to determine the killing effects of OM and its influence on cell cycle distribution, telomerase activity and the expressions of hTERT, c-myc, p53 and mad1 in SW1116 cells.

RESULTS: Oxymatrine exhibited dose-dependent killing effects on SW1116 cells and induced G1/G0-phase arrest. It suppressed the telomerase activity of the cells in a dose- and time-dependent manner. After OM administration, the expression of hTERT in the SW1116 cells decreased, those of p53 and mad1 increased, and the expression of c-myc was unchanged.

CONCLUSIONS: Oxymatrine has dose-dependent killing effects on SW1116 cells and its antineoplastic activity might be attributed to inhibition of telomerase activity by means of its effects on hTERT and the upstream regulating genes.

Article Published Date : Dec 31, 2004
Study Type : In Vitro Study
Additional Links
Substances : Sophora Flavescens : CK(826) : AC(444)
Diseases : Colon Cancer : CK(1974) : AC(1240)
Pharmacological Actions : Apoptotic : CK(10472) : AC(8099), Telomerase Inhibitor : CK(104) : AC(71)

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Key Research Topics

Substance

Sophora Flavescens

Disease

Colon Cancer

Pharmacological Actions

Apoptotic
Telomerase Inhibitor

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