Ficus carica latex: possible chemo-preventive, apoptotic activity and safety assessment. - GreenMedInfo Summary
LLatex: Possible Chemo-Preventive, Apoptotic Activity and Safety Assessment.
Iran J Pharm Res. 2020 ;19(3):231-240. PMID: 33680025
Hepatocellular carcinoma is the third cause of cancer-related mortality with the low 5-year survival in which more than 50 percent of patients have recurrent cancer within 2 years of treatment. The present study investigated the cytotoxicity and lethal dose ofL. (Figure) latex and phytochemical composition of effective fraction. Figure latex was collected in summer and 4 fractions of Figure latex were prepared. The cytotoxic effect of each fraction was studied and the most effective fraction was selected for apoptosis assay, acute toxicity study, and phytochemical analysis using column chromatography. The isolated compounds were identified byH-NMR,C-NMR, and mass spectroscopy. Chloroform fraction was the most effective fraction with the ICvalue of 0.219 and 0.748 mg/mL for HepG2 and NIH cell lines, respectively. Presence of cells in early apoptotic phase was documented by flow cytometry assay. Single dose administration of 2g/kg of fraction did not cause any death. Phytochemical analyses confirmed presence of lupeol acetate and lupeol palmitate in chloroform fractionThe present study revealed that the chloroform fraction is not only 3.4 times more toxic in HepG2 cell line but also has lowtoxicity which could be considered as a good candidate for a chemo-preventive agent.