Antimicrobial Effects against Oral Pathogens and Cytotoxicity ofExtract.
Plants (Basel). 2020 Jul 3 ;9(7). Epub 2020 Jul 3. PMID: 32635264
We aimed to evaluate the antimicrobial effects ofextract onandand its biocompatibility for dental applications. The antimicrobial activity of theextracts at concentrations of 50, 100, 150, and 200µg/mL was assessed using agar disk diffusion tests, counting the total number of colony-forming units (CFUs), spectrophotometric growth inhibitory assays, and microbial morphology observations using scanning electron microscopy (SEM; Merin, Carl Zeiss, Oberkochen, Germany). We measured the polyphenol and flavonoid contents ofextracts using ultraviolet-visible spectrometry and the cytotoxicity of these extracts using an MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. We identified thatextracts had significant antimicrobial effects againstand. The optical density of the experimental groups significantly decreased compared with that of the control group. SEM images revealed that theextract affected the morphology and density ofand. The extract concentration of flavonoids, but not polyphenols, increased with increasing concentrations of theextract. Furthermore, cell viabilities were more than 70% forextracts with concentrations of 50 and 100μg/mL. Naturally derivedis biocompatible and exhibits an excellent antimicrobial effect against oral pathogens such asand. Thus,extracts can be used for the development of oral products that treat and prevent oral diseases.