Abstract Title:

Effect of free radicals on the biological action of genistein in vitro and synergism with mitomycin C.

Abstract Source:

Anticancer Res. 2009 Aug;29(8):3179-83. PMID: 19661332

Abstract Author(s):

Johannes Hartmann, Nikola Getoff


BACKGROUND: The effect of oxidizing and reducing free radicals on the biological properties of genistein (GEN) were investigated in vitro. MATERIALS AND METHODS: MCF-7 cells were treated with GEN (5 micromol or 40 micromol) and/or mitomycin C (MMC; 2.5 micromol). For testing a possible radiosensitizing and synergistic effect of GEN in relation to MMC, a radiation dose of gamma-rays was applied up to 8 Gy. In order to produce the desired free radicals, the media were saturated with: (i) air (46% OH and 54% O2(*-)), (ii) N2O (90% OH, 10% H), or (iii) argon (44% e(-)(aq), 10% H and 46% OH). The survival fractions of the cells were determined by clonogenic assay. Results and CONCLUSION: GEN was found to be able to sensitize MCF-7 cells against the action of reducing (e(-)(aq), H, R(*)) as well as of oxidizing (OH, O2(*-)) free radicals. This ability was enhanced with increasing GEN concentration. Moreover, GEN acted synergistically with MMC in reducing as well as in oxidizing environments.

Study Type : In Vitro Study

Print Options

Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2021 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.