Ginsenoside Rg3 inhibits the biological activity of SGC-7901. - GreenMedInfo Summary
Ginsenoside Rg3 inhibits the biological activity of SGC-7901.
Food Sci Nutr. 2020 Aug ;8(8):4151-4158. Epub 2020 Jun 24. PMID: 32884696
Aim: To explore the suppressive effects of ginsenoside Rg3 on the biological activities of gastric cancer and the mechanisms responsible therein, by conducting an in vitro study.
Materials and Methods: SGC-7901 gastric cancer cells were divided into NC, DMSO, Gin-Low (10 mg/L), Gin-Middle (20 mg/L), and Gin-High (40 mg/L) groups. Using MTT, flow cytometry, transwell, and wound-healing assays, the cell biological activities in the different groups were evaluated; the protein expression levels of PTEN, p-PI3K, AKT, and P53 were measured by Western blot assay, andp-PI3K nuclear volume was evaluated by immunofluorescence.
Results: The SGC-7901 cell proliferation rate was depressed significantly, and cell apoptosis increased significantly while cells were arrested in the G1 phase ( < .05) with ginsenoside Rg3 treatment in a dose-dependent manner ( < .05). Meanwhile, the SGC-7901 cell invasion number and wound-healing rate of ginsenoside Rg3-treated groups were significantly downregulated compared with those of the NC group, also in a dose-dependent manner ( < .05). PTEN and P53 protein expression levels were significantly increased, and p-PI3K and AKT protein expression levels were significantly depressed in ginsenoside Rg3-treated groups in a dose-dependent manner ( < .05).
Conclusion: Ginsenoside Rg3 suppresses gastric cancer via regulation of the PTEN/p-PI3K/AKT pathway.