Hemp seed may be a valuable source of hypotensive peptides. - GreenMedInfo Summary
New ACE-Inhibitory Peptides from Hemp Seed (Cannabis sativa L.) Proteins.
J Agric Food Chem. 2017 Dec 6 ;65(48):10482-10488. Epub 2017 Nov 17. PMID: 29112398
Lara P Orio
A hemp seed protein isolate, prepared from defatted hemp seed meals by alkaline solubilization/acid precipitation, was subjected to extensive chemical hydrolysis under acid conditions (6 M HCl). The resulting hydrolysate was fractionated by semipreparative RP-HPLC, and the purified fractions were tested as inhibitors of angiotensin converting enzyme (ACE). Mono- and bidimensional NMR experiments and LC-MS analyses led to the identification of four potentially bioactive peptides, i.e. GVLY, IEE, LGV, and RVR. They were prepared by solid-phase synthesis, and tested for ACE-inhibitory activity. The IC50 values were GVLY 16± 1.5 μM, LGV 145 ± 13 μM, and RVR 526 ± 33 μM, confirming that hemp seed may be a valuable source of hypotensive peptides.