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Abstract Title:

Honeysuckle Aqueous Extracts InducedSuppress EV71 Replication and Pathogenesis In Vitro and In Vivo and Is Predicted to Inhibit SARS-CoV-2.

Abstract Source:

Viruses. 2021 Feb 16 ;13(2). Epub 2021 Feb 16. PMID: 33669264

Abstract Author(s):

Ying-Ray Lee, Chia-Ming Chang, Yuan-Chieh Yeh, Chi-Ying F Huang, Feng-Mao Lin, Juan-Ting Huang, Chang-Chi Hsieh, Jen-Ren Wang, Hsiao-Sheng Liu

Article Affiliation:

Ying-Ray Lee

Abstract:

Honeysuckle (Thunb) is a traditional Chinese medicine (TCM) with an antipathogenic activity. MicroRNAs (miRNAs) are small non-coding RNA molecules that are ubiquitously expressed in cells. Endogenous miRNA may function as an innate response to block pathogen invasion. The miRNA expression profiles of both mice and humans after the ingestion of honeysuckle were obtained. Fifteen overexpressed miRNAs overlapped and were predicted to be capable of targeting three viruses: dengue virus (DENV), enterovirus 71 (EV71) and SARS-CoV-2. Among them,was examined to be capable of targeting the EV71 RNA genome by reporter assay and Western blotting. Moreover, honeysuckle-inducedsuppression of EV71 RNA and protein expression as well as viral replication were investigated both in vitro and in vivo. We demonstrated thattargeted EV71 at the predicted sequences using luciferase reporter plasmids as well as two infectious replicons (pMP4-y-5 and pTOPO-4643). The suppression of EV71 replication and viral load was demonstrated in two cell lines by luciferase activity, RT-PCR, real-time PCR, Western blotting and plaque assay. Furthermore, EV71-infected suckling mice fed honeysuckle extract or inoculated withshowed decreased clinical scores and a prolonged survival time accompanied with decreased viral RNA, protein expression and virus titer. The ingestion of honeysuckle attenuates EV71 replication and related pathogenesis partially through the upregulation ofexpression both in vitro and in vivo. Our previous report and the current findings imply that both honeysuckle and upregulatedcan execute a suppressive function against the replication of DENV and EV71. Taken together, this evidence indicates that honeysuckle can induce the expression ofand that this miRNA as well as 11 other miRNAs have great potential to prevent and suppress EV71 replication.

Study Type : Animal Study, In Vitro Study

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Sayer Ji
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