Hydrogen-Rich Water Ameliorates Total Body Irradiation-Induced Hematopoietic Stem Cell Injury by Reducing Hydroxyl Radical.
Oxid Med Cell Longev. 2017 ;2017:8241678. Epub 2017 Jan 22. PMID: 28243358
We examined whether consumption of hydrogen-rich water (HW) could ameliorate hematopoietic stem cell (HSC) injury in mice with total body irradiation (TBI). The results indicated that HW alleviated TBI-induced HSC injury with respect to cell number alteration and to the self-renewal and differentiation of HSCs. HW specifically decreased hydroxyl radical (OH) levels in the c-kitcells of 4 Gy irradiated mice. Proliferative bone marrow cells (BMCs) increased and apoptotic c-kitcells decreased in irradiated mice uptaken with HW. In addition, the mean fluorescence intensity (MFI) of-H2AX and percentage of 8-oxoguanine positive cells significantly decreased in HW-treated c-kitcells, indicating that HW can alleviate TBI-induced DNA damage and oxidative DNA damage in c-kitcells. Finally, the cell cycle (P21), cell apoptosis (BCL-XL and BAK), and oxidative stress (NRF2, HO-1, NQO1, SOD, and GPX1) proteins were significantly altered by HW in irradiated mouse c-kitcells. Collectively, the present results suggest that HW protects against TBI-induced HSC injury.