Article Publish Status: FREE
Abstract Title:

Immune Modulation Effects ofVarietyon Enterocytes and Intestinal Stem Cells in a 5-FU-Induced Mucositis Mouse Model.

Abstract Source:

Gastroenterol Res Pract. 2021 ;2021:3068393. Epub 2021 Jan 25. PMID: 33564301

Abstract Author(s):

Chun-Yan Yeung, Jen-Shiu Chiang Chiau, Mei-Lien Cheng, Wai-Tao Chan, Szu-Wen Chang, Chuen-Bin Jiang, Hung-Chang Lee

Article Affiliation:

Chun-Yan Yeung


Background: Intestinal mucositis remains one of the most deleterious side effects in cancer patients undergoing chemotherapy. We hypothesize that the probiotics could preserve gut ecology, ameliorate inflammation, and protect epithelia via immune modulations of enterocytes and intestinal stem cells. Our aim is to characterize these changes and the safety of probiotics via a 5-fluorouracil- (5-FU-) induced intestinal mucositis mouse model.

Methods: 5-FU-injected BALB/c mice were either orally administrated with saline or probiotic suspension ofvariety(Lcr35). Diarrhea scores, serum proinflammatory cytokines, and T-cell subtypes were assessed. Immunostaining analyses for the proliferation of intestinal stem cells CD44 and Ki67 were processed. Samples of blood and internal organs were investigated for bacterial translocation.

Results: Diarrhea was attenuated after oral Lcr35 administration. Serum proinflammatory cytokines were significantly increased in the 5-FU group and were reversed by Lcr35. A tremendous rise of the CD3/CD8count and a significant decrease of CD3CD4/CD3CD8ratios were found in the 5-FU group and were both reversed by Lcr35. 5-FU significantly stimulated the expression of CD44 stem cells, and the expression was restored by Lcr35. 5-FU could increase the number of Ki67 proliferative cells. No bacterial translocation was found in this study.

Conclusions: Our results showed that 5-FU caused intestinal inflammation mainly via Th1 and Th17 responses. 5-FU could stimulate stem cells and proliferation cells in a mouse model. We demonstrate chemotherapy could decrease immune competence. Probiotics were shown to modulate the immune response. This is the first study to analyze the immune modulation effects and safety ofstrain on enterocytes and intestinal stem cells in a mouse model.

Print Options

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2022 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.