Immunomodulatory activity of puerarin in RAW264.7 macrophages and cyclophosphamide-induced immunosuppression mice.
Immunopharmacol Immunotoxicol. 2021 Feb 14:1-7. Epub 2021 Feb 14. PMID: 33583301
CONTEXT: Puerarin, a natural isoflavone extracted from, is famous for treating various cardiovascular and cerebrovascular diseases. However, little is known about its direct immunomodulatory activity.
OBJECTIVE: This study was designed to investigate theandimmunomodulatory effects ofby using the murine monocyte-macrophage cell line RAW264.7 and immunosuppressed cyclophosphamide-induced mice.
METHODS: MTT and neutral red phagocytosis assays were conducted to evaluate theimmunomodulatory activities of puerarin on cell viability and phagocytosis by measuring the proliferation, phagocytic, nitric oxide (NO) ability, and TNF-α production ability of stimulated and lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Immunosuppressed cyclophosphamide-induced mice were used to evaluate theimmunomodulatory activities of puerarin by measuring IL-4 and IFN-γ, the serum half hemolysis value, spleen and thymus index, and proliferation assay for splenic lymphocytes.
RESULTS AND DISCUSSION: Results showed that puerarin improves immunomodulatory activity by increasing cell proliferation, cell phagocytosis, and NO secretion in RAW264.7 macrophages and reduces the abnormal immunologic activity by decreasing cell phagocytosis and NO secretion in LPS-stimulated RAW264.7 macrophages. In addition, puerarin enhanced the immunologic activity of cyclophosphamide-induced immunosuppression mice by increasing the secretion of NO, IFN-γ, and IL-4, the serum half hemolysis value (HC), the spleen and thymus index, and proliferation for splenic lymphocytes.
CONCLUSION: Puerarin exhibited an upregulated immunomodulatory effect on RAW264.7 macrophages and immunosuppression mice. In addition, puerarin had a downregulated immunomodulatory effect on RAW264.7 macrophages. The results suggest that puerarin could be a promising immunomodulator to assist in the treatment of tumors.