Abstract Title:

Indole-3-carbinol enhances ultraviolet B-induced apoptosis by sensitizing human melanoma cells.

Abstract Source:

Cell Mol Life Sci. 2006 Nov;63(22):2661-8. PMID: 17086378

Abstract Author(s):

D-S Kim, Y-M Jeong, S-I Moon, S-Y Kim, S-B Kwon, E-S Park, S-W Youn, K-C Park


Indole-3-carbinol (I3C) has been found to act against several types of cancer, while ultraviolet B (UVB) is known to induce the apoptosis of human melanoma cells. Here, we investigated whether I3C can sensitize G361 human melanoma cells to UVB-induced apoptosis. We examined the effects of combined I3C and UVB (I3C/UVB) at various dosages. I3C (200 microM)/UVB (50 mJ/cm(2)) synergistically reduced melanoma cell viability, whereas I3C (200 microM) or UVB (50 mJ/cm(2)), separately, had little effect on cell viability. DNA fragmentation assays indicated that I3C/UVB induced apoptosis. Further results show that I3C/UVB activates caspase-8, -3, and Bid and causes the cleavage of poly(ADP-ribose) polymerase. Moreover, I3C decreased the expression of the anti-apoptotic protein, Bcl-2, whereas UVB increased the translocation of Bax to mitochondria. Thus, an increased Bax/Bcl-2 ratio by I3C/UVB may result in melanoma apoptosis. In conclusion, our study demonstrated that I3C sensitizes human melanoma cells by down-regulating Bcl-2.

Study Type : In Vitro Study
Additional Links
Therapeutic Actions : Light Therapy : CK(496) : AC(83)
Pharmacological Actions : Apoptotic : CK(6986) : AC(5304)

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