Article Publish Status: FREE
Abstract Title:

Indole-3-carbinol inhibits nasopharyngeal carcinoma cell growthandthrough inhibition of the PI3K/Akt pathway.

Abstract Source:

Exp Ther Med. 2014 Jul ;8(1):207-212. Epub 2014 Apr 25. PMID: 24944623

Abstract Author(s):

Cheng-Gang Mao, Ze-Zhang Tao, Zhe Chen, Chen Chen, Shi-Ming Chen, Li-Jia Wan

Article Affiliation:

Cheng-Gang Mao


Indole-3-carbinol (I3C) is an active component of cruciferous vegetables and has been shown to markedly inhibit the growth of a variety of tumors. However, the role of I3C in nasopharyngeal carcinoma (NPC) remains unclear. Thus, the aim of the present study was to investigate the inhibition of NPC cells by I3Cand. The human CNE2 NPC cell line was treated with various concentrations (0, 100, 200 and 300μM) of I3C and analysis of cell proliferation after 0, 24, 48 and 72 h, apoptosis after 48 h and expression levels of phosphatidylinositol 3-kinase (PI3K)/Akt pathway-associated proteinswas performed. BALB/c nude mice were divided into the following groups: Prevention, treatment and control., all the nude mice were inoculated with CNE2 NPC cells and the mice in the prevention and treatment groups were administered a diet containing 0.5% I3C prior to and following inoculation, respectively. The tumoricidal effect of I3C was investigated in the nude mice. After eight weeks, the expression levels of PI3K/Akt pathway-associated proteins were analyzed in the tumors from the nude mice in each group. The results demonstrated that with increasing I3C concentrations, cell proliferation decreased and apoptosis increased significantly. In addition, the expression levels of PI3K/Akt pathway-associated proteins decreased. In the animal experiments, the prevention and treatment groups developed smaller tumors and the expression levels of PI3K/Akt pathway-associated proteins were reduced when compared with those in the control group. In addition, very few changes to the heart, liver and kidney tissues were observed with hematoxylin and eosin staining in all the groups. Therefore, the results of the present study indicated that I3C inhibited the growth of NPC cells and induced apoptosis effectivelyand. The underlying mechanism may be that I3C suppresses the PI3K/Akt pathway.

Study Type : Animal Study, In Vitro Study

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