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Article Publish Status: FREE
Abstract Title:

Isorhamnetin Inhibits Human Gallbladder Cancer Cell Proliferation and Metastasis via PI3K/AKT Signaling Pathway Inactivation.

Abstract Source:

Front Pharmacol. 2021 ;12:628621. Epub 2021 Feb 17. PMID: 33679411

Abstract Author(s):

Tianyu Zhai, Xiaoyu Zhang, Zhenyu Hei, Longyang Jin, Chao Han, Audrey Tsznam Ko, Xiaofeng Yu, Jiandong Wang

Article Affiliation:

Tianyu Zhai

Abstract:

Gallbladder cancer (GBC) is the most common biliary tract tumor with a poor prognosis. Isorhamnetin is a flavonoid compound extracted fromand has several pharmacological effects including anti-inflammatory and anti-cancer properties. We treated GBC-SD and NOZ of GBC cell lines with different isorhamnetin concentrations. A cell counting kit-8 (CCK-8) assay, transwell assay, Hoechst 33342 stain assay, flow cytometric analysis, and a colony-forming assay were performed to investigate the effect of isorhamnetin on the proliferation, apoptosis, metastasis, and cycle arrest of GBC cells. A western blotting assay was conducted to explore the related protein expression level of GBC cells. A mice xenograft model and immunohistochemistry staining were employed to assess the effect of isorhamnetin. Isorhamnetin was found to suppress cell proliferation and metastasis, and trigger apoptosis and arrest the G2/M phase in GBC cells via the inactivation of the PI3K/AKT signaling cascade. Our findings are of clinical significance in providing a novel treatment approach for GBC.

Study Type : In Vitro Study

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