Article Publish Status: FREE
Abstract Title:

Attenuates-Induced Stress of Epithelial Cells by Modulating Tight-Junction Genes and Cytokine Responses.

Abstract Source:

Front Microbiol. 2018 ;9:1439. Epub 2018 Jul 2. PMID: 30013538

Abstract Author(s):

Alexia F P Lépine, Nicole de Wit, Els Oosterink, Harry Wichers, Jurriaan Mes, Paul de Vos

Article Affiliation:

Alexia F P Lépine


Salmonellosis is a prevalent food-borne illness that causes diarrhea in over 130 million humans yearly and can lead to death. There is an urgent need to find alternatives to antibiotics as many salmonellae are now multidrug resistant. As such, specific beneficial bacteria and dietary fibers can be an alternative as they may preventTyphimurium (STM) infection and spreading by strengthening intestinal barrier function.We tested whether immune active long-chain inulin-type fructans and/orW37,W63, andW56 can strengthen barrier integrity of intestinal Caco-2 cells in the presence and absence of a STM. Effects of the ingredients on intestinal barrier function were first evaluated by quantifying trans-epithelial electric resistance (TEER) and regulation of gene expression by microarray. Onlyhad effects on TEER and modulated a group of 26 genes related to tight-junctions. Inulin-type fructans,W63 andW56 regulated other genes, unrelated to tight-junctions.also had unique effects on a group of six genes regulating epithelial phenotype toward follicle-associated epithelium.W37 was therefore selected for a challenge with STM and prevented STM-induced barrier disruption and decreased secretion of IL-8.W37 increases TEER and can protect against STM induced disruption of gut epithelial cells integrity. Our results suggest that selection of specific bacterial strains for enforcing barrier function may be a promising strategy to reduce or prevent STM infections.

Study Type : In Vitro Study

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